Direct and noninvasive fluorescence analysis of an RNA-protein interaction based on a CRISPR/Cas12a-powered assay

Xueliang Wang, Shaozhen Jing,Wanhe Wang,Jing Wang

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy（2023）

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摘要

We report the first fluorescence biosensor for the detection of RNA-protein interactions based on the CRISPR/Cas12a system, in which crRNA also serves as a RNA aptamer for a RNA-bounding protein, enabling direct and noninvasive analysis.

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关键词

CRISPR/Cas12a, RNA-protein interactions, RNA aptamer, Fluorescence, VEGF165

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