Release of chimeras and efficient selection of editing mutants by CRISPR/Cas9-mediated gene editing in apple

Furong Li,Natsumi Kawato,Haruka Sato,Yasuyuki Kawaharada,Mitsuki Henmi,Ami Shinoda,Taichi Hasunuma,Chikako Nishitani,Yuriko Osakabe,Keishi Osakabe,Masato Wada,Norimitsu Tanaka,Manabu Watanabe,Chunfen Zhang,Shu Deng,Sadao Komori

Scientia Horticulturae（2023）

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摘要

•PDS knockout variant of the apple cultivar "Greensleeves" was observed in 6th month.•Chimeras can be released by 2 to 3 times subculture to obtain albino plants.•"Additional regeneration" of green transformant regenerated mutant at high frequency.•New mutations were generated simultaneously during the "Additional regeneration".

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关键词

Additional regeneration, Chimerism, CRISPR/Cas9 knockout, PDS, Selection, Transformant

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