Evaluation of four commercial ELISAs to measure tissue factor in human plasma

Ana T.A. Sachetto,Sierra J. Archibald,Ravi Bhatia,Dougald Monroe,Yohei Hisada,Nigel Mackman

Research and Practice in Thrombosis and Haemostasis（2023）

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摘要

•Tissue factor (TF) in human plasma can be measured using activity- or antigen-based assays.•We evaluated the ability of 4 commercial ELISAs to measure TF in human plasma.•Two of the 4 ELISAs detected recombinant TF spiked into human plasma.•One ELISA detected TF in plasma from leukemia patients with high levels of extracellular vesicle TF activity.

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关键词

acute leukemia, coagulation, enzyme-linked immunosorbent assay, extracellular vesicles, plasma, tissue factor

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