Fabrication of metal-organic framework based electrochemical Leishmania immunosensor
Microchemical Journal(2023)
Abstract
Leishmaniasis is a disease with a high impact on public health in many countries. In this study, an electro-chemical immunosensor for Leishmania major surface protease (Gp63) antibody detection was developed using copper metal organic framework (Cu-(NH2-BDC)-MOF) modified gold screen printed electrode (AuSPE). For this purpose, after modification step of AuSPE with Cu-(NH2-BDC)-MOF, recombinant L.donovani antigen was immobilized on to electrode surface via crosslinker where change in electrochemical signal was monitored with electrochemical impedance spectroscopy technique. After conducting comprehensive optimization studies on experimental parameters of developed Leishmania immunosensor, analytical characteristics were searched. As a result, it was observed that anti-gp63 solution diluted up to the ratio of 1:1500 could be detected with the developed sensor. Meanwhile, relative standard deviation value was found as 4.67% for anti-gp63 diluted in the ratio of 1:80 (n = 3). Accordingly, real sample analysis was also performed by using Leishmania parasite crude antigen and rabbit serum which were positive and negative control samples, respectively. According to the re-sults obtained, it can be concluded that a practical immunosensor that can allow the early diagnosis of leish-maniasis was developed.
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Key words
Leishmania major surface protease (Gp63),Recombinant L,donovani antigen (KMP-11),Metal organic framework,Impedimetric biosensor
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