qKW9encodes a pentatricopeptide repeat protein affecting photosynthesis and grain filling in maize

AbstractKernel weight is an important yield component in maize that was selected during domestication. Many kernel weight genes have been identified through mutant analysis, and are mostly involved in the biogenesis and functional maintenance of organelles or other fundamental cellular activities. However, only a limited number of loci underlying quantitative variation in kernel weight have been cloned. Here we characterize a maize kernel weight QTL,qKW9and find that it encodes a DYW motif pentatricopeptide repeat protein involved in C-to-U editing of NdhB, a subunit of the chloroplast NADH dehydrogenase-like complex. In a nullqKW9background, C-to-U editing of NdhB was abolished, and photosynthesis was reduced, suggesting thatqKW9regulates kernel weight by controling the maternal source of photosynthate for grain filling. Characterization ofqKW9highlights the importance of optimizing photosynthesis on maize grain yield production.