An integrated approach to evaluate acetamiprid-induced oxidative damage to tRNA in human cells based on oxidized nucleotide and tRNA profiling

•An integrated method was developed to study tRNA oxidative damage.•Acetamiprid induced the increment of ROS, oxidized nucleotides, and DNA/RNA damage.•Developing a TMSD labeling-based LC-MS/MS method to quantify oxidized nucleotides.•Investigating acetamiprid-caused 8-oxo-G accumulation and damage sites in tRNA.