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Molecular background of HAC1-driven improvement in the secretion of recombinant protein in Yarrowia lipolytica based on comparative transcriptomics

Paulina Korpys-Woźniak,Ewelina Celińska

Biotechnology Reports（2023）

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Abstract
•HAC1 overexpression decreases intracellular r-Prot retention and enhances secretion.•HAC1 unconventional splicing rate was counted based on transcripts sequencing.•Implication of transcription factor Xbp1 and ligase Trl1 in Yarrowia UPR is proposed.
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Key words
Secretion,Heterologous protein,Yeast,Endoplasmic reticulum (ER) stress,Recombinant protein production
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