Roles and functions of exosomal miRNA in abdominal aortic aneurysm

Abstract Background and objective: abdominal aortic aneurysms（AAAs）are the permanent dilatation of the abdominal aorta, ruptured AAA is a serious threat to the patient's life. It’s hardly known about exosomal miRNAs in AAA. The main purpose of this article is to screen miRNAs which differentially expressed in exosomes from normal people and patients with AAA, and to understand the mechanism of work.Material and methods: The plasma of healthy control group and patients with AAA were collected, and the RNAs from exosomes was isolated and sequenced. The mature miRNA sequence of miRBase 21 database was used to identify the known type and expression of miRNAs. DEGseq software was used to analyze the types of miRNAs with significant difference between the experimental group and the control group ( P ＜ 0.05, | log 2 (FoldChange) | ≥ 1 ). The targets of miRNAs were detected by miRTarBase, miRDB, TargetScan and miRWalk software. Targets were analyzed based on Kyoto encyclopedia of genes and genomes（ KEGG ）biological pathway and gene ontology（ GO ）functional enrichment analysis. Conclusion: miRNAs in exosomes regulate in the progress of AAA by activating PI3K-Akt/mTOR and MAPK pathway, and its mechanism needs more research. Background and objective: abdominal aortic aneurysms（AAAs）are the permanent dilatation of the abdominal aorta, ruptured AAA is a serious threat to the patient's life. It’s hardly known about exosomal miRNAs in AAA. The main purpose of this article is to screen miRNAs which differentially expressed in exosomes from normal people and patients with AAA, and to understand the mechanism of work. Material and methods: The plasma of healthy control group and patients with AAA were collected, and the RNAs from exosomes was isolated and sequenced. The mature miRNA sequence of miRBase 21 database was used to identify the known type and expression of miRNAs. DEGseq software was used to analyze the types of miRNAs with significant difference between the experimental group and the control group ( P ＜ 0.05, | log 2 (FoldChange) | ≥ 1 ). The targets of miRNAs were detected by miRTarBase, miRDB, TargetScan and miRWalk software. Targets were analyzed based on Kyoto encyclopedia of genes and genomes（ KEGG ）biological pathway and gene ontology（ GO ）functional enrichment analysis.Conclusion: miRNAs in exosomes regulate in the progress of AAA by activating PI3K-Akt/mTOR and MAPK pathway, and its mechanism needs more research.