Development of nano-emulsions based onAyapana triplinervisfor the control ofAedes aegyptilarvae

AbstractAyapana triplinervisis a plant species used in traditional medicine and in mystical-religious rituals by traditional communities in the Amazon. The aim of this study is to evaluate the larvicidal activity againstA. aegyptiof nano-emulsions containing essential oils fromA. triplinervismorphotypes, and acute oral toxicity in non-target organism. Essential oils were identified and nano-emulsions were prepared using the low energy method. The mortality test ofA. aegyptilarvae was performed according to the protocol recommended by the World Health Organization, and toxicity in non-target mammals was performed according to the OECD. Phytochemical analyses indicated the major compounds (E)-Caryophyllene (45.93%) and Thymohydroquinone Dimethyl Ether (32.93%) in morphotype A, and in morphotype B, Thymohydroquinone Dimethyl Ether (84.53%) was found. Morphotype A essential oil nano-emulsion showed a particle size of 101.400 ± 0.971 nm (PdI = 0.124 ± 0.009 and ZP = −19,300 ± 0.787 mV). Morphotype B essential oil nano-emulsion had a particle size of 104.567 ± 0.416 nm (PdI = 0.168 ± 0.016 and ZP = −27,700 ± 1,307 mV). Histomorphological analyses showed the presence of inflammatory cells in the liver of animals treated with morphotype A essential oil nano-emulsion (MAEON) and morphotype B essential oil nano-emulsion (MBEON). Congestion and the presence of transudate with leukocyte infiltration in the lung of animals treated with MAEON were observed. The nano-emulsions containing essential oils ofA. triplinervismorphotypes showed an effective nanobiotechnological product in the chemical control ofA. aegyptilarvae and safe for non-target mammals.