不同有机添加物对蝴蝶兰原球茎增殖的影响

Li Ruting,Gong Zihui, Zhang Yingjie,Sun Jixia,Guo Wenjiao,Zhang Jingwei,Liu Shuhe,Pan Xiangjun,Liu Xueqing

Journal of Agricultural Sciences（2020）

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Abstract

为了探究不同有机添加物对蝴蝶兰原球茎组培快繁的影响,将♀"大辣椒"×♂"粉圆唇"杂交育种后代无菌播种后的原球茎接种在1/2 MS+6-BA 4.0 mg/L+NAA 2.0 mg/L+不同有机添加物+活性炭0.5 g/L+琼脂5.5 g/L+蔗糖10 g/L的培养基上.结果表明:原球茎增殖效果最好的是添加200 mL/L椰汁的培养基,平均增殖倍率为6.4;其次为马铃薯,平均增殖倍率为5.3;最佳培养周期为60 d.

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