Selective plane activation structured illumination microscopy
Nature Methods(2023)
摘要
Resolution enhancement in structured illumination microscopy is hindered when volumetric samples are observed owing to background signals from out-of-focus planes. In this study, we utilized selective plane illumination and reversibly photoswitchable fluorescent proteins to realize structured illumination within the focal plane and eliminate the out-of-focus background. We demonstrated high-resolution fluorescence imaging of volumetric samples, including dense fluorescent objects in a cell spheroid.
### Competing Interest Statement
K. T., R. O., and K. Fujita filed a patent application (May 26, 2023) for the proposed method.
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