Chromosome-scale Genome Assembly of the Allopolyploid Arundo donax

Arundo donax L ( A. donax ). is a promising energy crop with high biomass and wide adaptability, while lack of reference genome limiting the genetic improvement of this crop. Here, we report two different chromosome-scale assemblies of A. donax genome (0004 and 0408) using Pacbio SMRT sequencing and Hi-C technology. The genome size of these two assemblies is 1.30 Gb / 2.86 Gb with contig N50 33.15 Mb / 34.62 Mb respectively. A total of 74,403 / 167,586 gene models were predicted in 0004 and 0408 genome, of which over 90% of genes were functionally annotated. Comparative genome analysis revealed that 0004 is alloenneaploid and 0408 is allohexaploid. Further analysis revealed that A. donax undergone strong gene family expansion and two whole-genome duplication events during evolution. Our results will enhance genetic understanding and promote the genetic improvement of A. donax . ### Competing Interest Statement The authors have declared no competing interest.