Importin α as a molecular marker for investigating the microenvironment of micronuclei
bioRxiv (Cold Spring Harbor Laboratory)(2023)
摘要
Micronuclei (MN) are nuclear membrane-enclosed cellular structures that contain DNA/chromatin fibers that can lead to genomic instability. Here, we report that importin α, known as the nuclear transport factor, is highly concentrated in some of the MN in cultured human cancer cells. We observed that importin α1 accumulated in the MN together with other subtypes, indicating that this represented a conserved feature of the importin α family. Indirect immunofluorescence analysis revealed that the MN localization of importin α1 was regulated independently of canonical nuclear transport-related factors such as importin β1, CAS/CSE1L, nucleoporins, and lamins. In contrast, importin α1 signals merged with histone H3 modified by trimethylation at lysine 4 in MN, indicating that importin α1 was associated with euchromatin in MN. Furthermore, we found a mutually exclusive relationship between importin α1 and RAD51, a homologous recombination repair protein, in relation to MN accumulation in cells exposed to etoposide, a DNA damaging reagent. Our findings suggest that importin α is a previously overlooked molecular marker for assessing the microenvironment and quality control of MN in human cancer cells.
Summary statement Accumulation of importin α in micronuclei, followed by alteration of their microenvironment, suggests the non-canonical function of importin α in genome instability and cancer development.
### Competing Interest Statement
The authors have declared no competing interest.
更多查看译文
关键词
micronuclei,microenvironment,molecular marker
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要