ElecFeX: A user-friendly toolkit for efficient feature extraction from single-cell electrophysiological recordings

Motivation Profiling neurons by their electrophysiological phenotype is essential for understanding their roles in information coding within and beyond the nervous systems. Technological development has unleashed our power to record neurons more than ever before, yet the booming size of the dataset poses new challenges for data analysis. Current software tools require users to have either significant programming knowledge or to devote great time and effort, which impedes their prevalence and adoption among experimentalists. To address this problem, here we present ElecFeX, a MATLAB-based graphical user interface designed for a more accessible and efficient analysis of single-cell electrophysiological recordings. ElecFeX has a simple and succinct graphical layout to enable effortless handling of large datasets. This tool includes a set of customizable methods for most common electrophysiological features, and these methods can process multiple files all at once in a reliable and reproducible manner. The output is assembled in a properly formatted file which is exportable for further analysis such as statistical comparison and clustering. By providing such a streamlined and user-friendly open-sourced interface, we hope ElecFeX can benefit broader users for their studies associated with neural activity. Summary Characterizing neurons by their electrophysiological phenotypes is essential for understanding the neural basis of behavioral and cognitive functions. Recent developments in electrode technologies have enabled the collection of hundreds of neural recordings; that necessitated the development of new toolkits capable of performing feature extraction efficiently. To address this urgent need for a powerful and accessible tool, we present ElecFeX, an open-source MATLAB-based toolbox that (1) has a succinct and intuitive graphical user interface, (2) provides generalized methods for wide-ranging electrophysiological features, (3) processes large-size dataset effortlessly, and (4) yields formatted output for further analysis such as neuronal characterization and classification. We implemented the toolbox on a diverse set of neural recordings and demonstrated its functionality, efficiency, and versatility in capturing features that can well-distinguish neuronal subgroups across brain regions and species. ElecFeX is thus presented as a powerful tool to significantly promote future studies on neuronal electrical activity. ### Competing Interest Statement The authors have declared no competing interest.