The Effect and Mechanism of ICA Regulation of miR-122-5p which from Osteoblasts-Derived Exosomes on Osteogenesis and Migration of BMSCs

Abstract BackgroundAvascular necrosis of femoral head（ANFH）is a common disease in orthopedics, which seriously affects the quality of life of patients. At present, there is no clear method to solve it in clinical practice. In order to cope with this worldwide problem, many scholars have devoted themselves to the research on the treatment of ANFH. Promoting the osteogenic differentiation and directed migration of stem cells is one of the main ideas for the treatment of ANFH. In recent years, more and more researches have begun to pay attention to Chinese medicine and Chinese medicine extracts. At present, in the field of bone regeneration, epimedium is the most in-depth study of Chinese herbal medicine, and Icariin is one of the main active ingredients of epimedium. MethodsDifferent concentrations of Icariin were applied to bone marrow mesenchymal stem cells (BMSCs), and the optimal concentration of Icariin to promote osteogenic differentiation and migration of BMSCs was observed. Then the exosomes from osteoblasts were combined with the optimal concentration of Icariin to BMSCs, observe the effect of promoting osteogenic differentiation and migration of BMSCs. Using high-throughput sequencing, analyze the composition of osteoblast-derived exosomes, predict the target miRNA, and then use miRNA minic and inhibitor to verify. ResultsThe optimal concentration of Icariin to promote osteogenic differentiation and migration of BMSCs is 1×10-7 M. Icariin combined with osteoblast-derived exosomes can effectively promote the osteogenic differentiation and migration of BMSCs. The top four miRNAs which content in osteoblast-derived exosomes are let-7a-5p, miR-100-5p, miR-21-5p, miR-122-5p, and analysis shows that miR-122-5p is related to osteogenic differentiation , The verification found that miR-122-5p can significantly promote the osteogenic differentiation and migration of BMSCs. ConclusionsIcariin can clearly promote the osteogenic differentiation and migration of BMSCs, and osteoblast-derived exosomes can also effectively promote the osteogenic differentiation and migration of BMSCs, the combination of Icariin and osteoblast-derived exosomes can enhance this effect. In this process, one of its targets is miR-122-5p.