最新PCR技术及其在妇产科的应用
MODERN JOURNAL OF INTEGRATED TRADITIONAL CHINESE AND WESTERN MEDICINE(2005)
Abstract
PCR技术即聚合酶联反应(polymerase chain reaction)是体外利用酶促反应扩增DNA或者RNA片段的技术.常规PCR的原理为将待扩增的核酸(模板)与引物、四种脱氧核苷酸和DNA聚合酶等混合在一起,经过高温使模板2条链分开(变性),然后降温(退火)使引物与模板结合,再升温到DNA聚合酶的最适温度,使四种脱氧核苷酸在引物的3'端延伸,使模板DNA链增加1倍.经过25~35次循环,将在引物之间的模板DNA得到指数倍的扩增.
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