Autologous Platelet Lysate Could Have Role in Enhancing the Fracture Healing in Rabbits

Abstract Although bone substitutes supplemented with platelet derived products are widely used in bone regeneration, the role of Platelet lysate needs to be further elucidate in treatment of bone damages. Accordingly, the aim of this animal study was to evaluate the benefits of autologous PL (APL) in fracture healing. Rabbit APL obtained from freeze-thawed platelet suspension and used for treatment of femoral bone damage in 20 rabbits after surgery procedure. Twenty other rabbits with femoral bone damage were assigned to control group which received Phosphate-buffered saline as therapy. ELISA and real-time PCR used for investigation of the secretion and mRNA expression of IL-1, IL‐6, TNF‐α and TGF‐β cytokines, respectively. The mRNA expression of several other related genes including MMP-3, MMP-13, IGF, FGF, VEGF and PDGF was also evaluated in days 0, 15, 30 and 60 which was higher in APL-treated rabbits than that of controls. However, secretion of pro-inflammatory cytokines was meaningfully lower than the control group and reduced significantly after two weeks. These observations indicated that factors released from PL and regulation between them could result in bone fracture healing.