Study on the Anti-Aging Physiological Characteristics and Molecular Mechanism of Camellia Oleifera

Abstract To study the physiological and molecular regulating mechanism of ancient Camellia oleifera which kept a exuberant vitality for more than one hundred years, leaves of 30a year old and > 100 year old Camellia oleifera were selected as targets. On the basis of the study of the generation and the clearance of reactive oxygen species, sequencing analysis of the transcriptome and expression profiling by high throughput sequencing analysis technique was conducted to study differentially expressed functional genes related to the tree age. It showed that the chlorophyll content and enzyme activities increased in ancient Camellia oleifera leaves. Expression of chlorophyll a/b binding protein gene, auxin related gene, the signal transduction factor and the transcription factor gene in ancient trees were all higher than mature tree. The down regulated gene expression of inductive genes related to protein degradation in ancient tree. Under the comprehensive function of those factors, ancient Camellia oleifera leaves still kept an exuberant vitality which was very useful for studies of stress resistance molecular biology and genetic improvement of Camellia oleifera.