Clinicopathological Significance of Up-Regulated Pir-1366 in NSCLC and its Effect on Tumor Migration and Metastasis

Abstract Background: piRNAs are a kind of noncoding RNAs that involve in tumorigenesis and development, but the role of piRNAs in NSCLC remain unclear. In this study, we explored the role of piR-1366 in NSCLC .Methods:The identification of piRNAs was performed using Human Arraystar piRNA Array in NSCLC tissues and adjacent normal tissues. up-regulated piR-1366 were validated in the NSCLC and selected for further study of migration and metastasis. In addition, the expression of piR-1366 in 87 cases of NSCLC was detected by in situ hybridization and the correlation between piR-1366 expression and pathological parameters of NSCLC patients was analyzed. meanwhile, bioinformatics, qRT-PCR and West blotting were used to examine the signal pathway of piR-1366 in migration. Results: piR-1366 was up-regulated and positive expression in NSCLC ,The positive expression of piR-1366 was closely related to lymph node metastasis of NSCLC, but not with age, gender, tumor location, tumor size, tumor grade and TNM stage. piR-1366 is transfected into lung cancer cells to promote its migration and metastasis. Mechanistically,WT1-3'UTR was complementary combination with piR-1366 by bioinformatic prediction and identified as a direct target of piR-1366 through dual-luciferase reporter assay. Over-expression and knockdown of WT1 could respectively rescue and simulate the effects induced by piR-1366. Finally, piR-1366 promoted migration and metastasis by the WT1 /CDH1 pathway in lung cancer cells. Conclusions:Thus,our article suggested that piR-1366 was a novel pro-metastasis oncogene and may represent a novel marker of diagnosis and treatment for Metastatic NSCLC.