A New Sight In The Key Prognosis-Related Proto-Oncogene FYN In Hepatocellular Carcinoma Based On WCGNA Hub-Gene Screening Strategy

Abstract Background: Hepatocellular Carcinoma (HCC) is the third deadly cancer worldwide. More breakthroughs are needed in the clinical practice of liver cancer, and new treatment strategies are required to carry out to benefit patients. This study aims to screen out other significant differences in genes associated with LIHC and analyze its prognostic value further. Methods: Here, we use the TCGA-LIHC database and the profiles of GSE25097 from GEO to explore the differential co-expression genes in HCC tissues compared with para-tumor (or healthy) tissues. Then, we utilized WGCNA to screen out differential co-expression genes. Finally, we explored the function of FYN in HCC cells and xenograft tumor models.Results: We identified ten hub genes in the protein-protein interaction (PPI) network, but only three (COLEC10, TGFBR3, and FYN) of them seem to have a close-related to the prognosis. The expression of FYN was negatively correlated with the prognosis of HCC patients. The xenograft model showed that overexpression of FYN could significantly inhibit malignant tumor behaviors and promote tumor cell apoptosis.Conclusion: Thus, FYN may be central to the development of LIHC and maybe a novel biomarker for clinical diagnosis and treatment.