Intrapuparial development and age estimation of Sarcophaga peregrina (Diptera: Sarcophagidae) for postmortem interval estimation

Sarcophaga peregrina Robineau-Desvoidy, 1830 (Diptera: Sarcophagidae) is a widely distributed species in the Palearctic, Oriental and Australian regions. Numerous studies and case reports have confirmed the value of this species in forensic investigations, but methods for estimating the age of the intrapuparial period of this species are lacking. In this study, S. peregrina pupae were sampled at 16 degrees C, 19 degrees C, 22 degrees C, 25 degrees C, 28 degrees C, 31 degrees C, and 34 degrees C. Morphological changes within the puparium were observed under a stereomicroscope. The results of the study showed that S. peregrina could complete its intrapuparial development at each of the seven tested constant temperatures. As the temperature increased, the average minimum duration of the intrapuparial period of S. peregrina decreased from 706.7 +/- 28.1 h at 16 degrees C to 170.7 +/- 4.6 h at 34 degrees C. The intrapuparial morphological changes of S. peregrina can be divided into 12 sub-stages. While some structures such as the compound eyes, mouthparts, antennae, thorax, legs, wings, and abdomen can be used individually to estimate intrapuparial age, we observed all these structures with local magnification photographs and classified the developmental process of these structures into 6-11 stages. The time range for the appearance of each sub-stage or structure was recorded in detail. The results of this study provide important basic data for estimating the minimum postmortem interval using the intrapuparial morphology of S. peregrina.