Structural properties, anti-fatigue and immunological effect of low molecular weight peptide from Monkfish

Monkfish (Lophius litulon) is a nutritious marine product that has attracted increasing attention. In this study, we demonstrated that the monkfish protein hydrolysate (MPH) administration could significantly prolonged the exhaustive swimming time of mice, and increased the hepatic glycogen (HG) level, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxides (GSH-PX) activities; in addition, markedly decreased the blood urea nitrogen (BUN), blood lactic acid (BLA), malonaldehyde (MDA) levels and lactate dehydrogenase (LDH) activity. Furthermore, the MPH administration also enhanced the proliferation of spleen lymphocytes and the activity of natural killer (NK) cells in mice. Besides, 43 peptides from MPH that rich in Phe, His, Ile, Leu and Thr were identified. Finally, 1245 of differentially expressed genes (DEGs) in NC vs MPH-H groups were obtained by transcriptome analysis, and GO classification and enrichment of KEGG pathway were studied. In conclusion, MPH treatment had the anti-fatigue and immunological effects in mice.