miR-212-3p Promotes Synovial Inflammation in Osteoarthritis by Regulating 11-Hydroxysteroid Dehydrogenase 1/Glucocorticoid/Glucocorticoid Induced Leucine Zipper Signaling Axis in Human Synovial Fibroblasts

To investigate the regulatory effect and mechanism of miR-212-3p-mediated 11 beta-HSD1/GC/GILZ signal axis on the inflammatory response of human synovial fibroblasts (HFLS). HFLS cells were cultured and divided into Control group, inhibitor-NC group and miR-212-3p group. Cell activity, apoptosis, inflammatory factors and 11 beta-HSD1/GC/GILZ signal axis expression were observed in each group. After inhibiting the expression of miR-212-3p, the HFLS cell inflammation model constructed by IL-1 beta increased cell activity, decreased apoptosis rate, decreased expression of inflammatory factors, and increased expression of 11(beta-HSD1, cortisol and GILZ. In conclusion, miR-212-3p targeted down-regulated the expression of 11 beta-HSD1. Inhibition of miR-2123p can regulate the 11 beta-HSD1/GC/GILZ signaling axis to promote the reversal of IL-1 beta-mediated HFLS cell inflammatory response.