Characteristics of the Chloroplast Genome of Adinandra bockiana and Comparative Analysis with Species of Pentaphylacaceae Family

Adinandra bockiana E. Pritz. ex Diels is a potential plant of the Adinandra genus that contains bioactive. This study presents the results of assembly and annotation of the chloroplast (cp) genome of A. bockiana , a comparison of it with Adinandra cp genomes of several other species, and proposed candidate DNA barcoding from the cp genome. The A. bockiana cp genome was reconstructed by raw reads from total DNA, assembling reads (each read is 150 bp in length) into contigs, and then the contigs were assembled again to sequence the complete cp genome. The A. bockiana cp genome is 156,284 bp in length and consists of a large single-copy (LSC) region of 85,693 bp, a small single-copy (SSC) region of 18,411 bp, a pair of inverted repeats (IRa and IRb) of 26,090 bp each, 129 functional genes, 51 SSRs, and other 70 repeat structures. The functional genes of the A. bockiana cp genome contain 84 protein-coding genes, 37 transfer RNA genes, and eight ribosomal RNA genes. A comparative analysis of the A. bockiana cp genome with the rest of the public Adinandra genomes showed that the number of genes and their structure were highly conserved in the surveyed group. Based on complete cp genomes and matK and trnL sequences of some species of the Adinandra and other genera, molecular phylogenetic trees were established which show the genetic relationships of the species belonging to the Pentaphylacaceae family. Among the chloroplast genes of A. bockiana , the matK and trnL genes are better candidates for phylogenetic analysis.