DNA markers in analysis of genetic diversity of Curcuma longa L. from Meghalaya

DNA markers viz. DAMD, ISJ and SRAP were used to characterize intra-specific genetic diversity of Curcuma longa (turmeric) collections from Meghalaya to evince levels of molecular variability and genetic diversity among the genotypes. Comparison of the degree of polymorphism and efficacy of the different molecular markers showed a high degree of polymorphism (> 93%) and high number of effective alleles (> 1.9) reflecting a relatively high genetic diversity among turmeric collections. UPGMA clustering pattern of DAMD, ISJ and SRAP revealed a mixed clustering pattern forming three major clusters. Overall, DNA-based markers used in the study were found to be efficient and highly informative. However, ISJ markers are more promising and can be considered as important tools for a better understanding of genetic relationships among the accessions and/or germplasm, and in selecting unique gene-pool for further biotechnological innovation and exploitation of C. longa germplasm.