Genomic and RNA-Seq Profiling of Patients with Heart Failure Identified Alterations in Phosphorylation and Immune Signaling Pathways

Abstract Background: Heart failure (HF) is a complex pathophysiological state in which the delivery of blood and nutrients to the tissues is inadequate. It is rarely curable and often associated with poor prognosis. The current study aimed to analyze the exomic and RNA-Seq data of patients with HF to identify the key altered pathways. Methods: Heart failure participants were recruited, and whole blood samples were collected. The samples were used for whole exome sequencing and RNA-Seq analysis. Gene expression and RNA-Seq results were verified by Gene chips and RT-PCRResults: We report a dysregulation of phosphorylation and immune signaling in patients with HF both by exomic and RNA-Seq data. We identified mutations in TITIN,OBSCURIN, NOD2, CDH2, MAP3K5, and SLC17A4 to be associated with HF by exomic analysis. And certain genes, S100A12, S100A8, S100A9, PFDN5, and TMCC2, were found upregulated in patients with HF by RNA-Seq. Conlcusion: Our results demonstrated the overall disruption of key phosphorylation and immune signaling pathways in patients with HF.