Influencing Factors of HLA-DR Expression in Orbital Preadipocytes of Patients With Thyroid Associated Ophthalmopathy

Abstract Purpose: To investigate the effects of IFN-γ, IL-6, PPAR-γ agonist pioglitazone and PPAR-γ antagonist GW9662 on preadipocyte proliferation and HLA-DR expression.Methods: Preadipocytes were cultured from thyroid associated ophthalmopathy patients and health ocular trauma patients and induced to differentiate in vitro. IFN-γ, IL-6, PPAR-γ agonist pioglitazone and PPAR-γ antagonist GW9662 were added to preadipocytes to observe their effects on proliferation and HLA-DR expression.Results: IFN-γ significantly increased the levels of HLA-DR mRNA and protein in preadipocytes which are cultured from thyroid associated ophthalmopathy patients. IFN-γ also reduced the accumulation of lipid in the cytoplasm of preadipocytes. On the contrary, IL-6 had no effect on the expression of HLA-DR. Pioglitazone increased the expression of HLA-DR in orbital preadipocytes of TAO patients, and promoted the proliferation and intracellular lipid accumulation of preadipocytes. On the contrary, GW9662 decreased the expression of HLA-DR in orbital preadipocytes of TAO patients, and reduced the proliferation and intracellular lipid accumulation of preadipocytes.Conclusion: IFN-γ increase the expression of HLA-DR in TAO orbital preadipocytes after differentiation. PPAR - γ antagonists may be beneficial to the treatment of TAO.