MiniXT protocol v1

We present the ‘mini-XT’ miniaturized tagmentation-based library preparation protocol used for Illumina WGS of SARS-CoV-2 positive samples. Reverse transcription and amplification is based upon the nCoV-2019 sequencing protocol v3 (LoCost)V.3 by Josh Quick. The key new feature of the protocol is the use of acoustic liquid transfer to automate and reduce volumes during library preparation. It is optimized for the sequencing of 384 samples, offering reduced consumable use and costs and improved throughput.