Lysosome immunopurification (LysoIP) protocol for subcellular metabolite profiling v1

Lysosomes function as metabolic hub in the cell by degrading and recycling biomolecules. Despite its critical cellular function, there has been challenges to quantitatively assess lysosomal metabolite profiles. To overcome this hurdle, we developed a rapid harvesting and purification method using immunoprecipitation (LysoIP). This protocol provides details for preparing LysoIP samples for metabolite profiling.