Prominin-1 (PROM1) Significantly Correlated With Bone Metastasis and Influenced Prognosis in Breast Cancer

Abstract BackgroundThis study aimed to explore the important biomarker associated with bone metastasis (BM) in breast cancer (BRCA).MethodsThe GSE175692 dataset was used to detect significant differential expressed genes (DEGs) between BRCA samples with or without BM, and important pathways were then explored. Further, we constructed protein-protein interaction (PPI) network on GEGs and filtered 5 vital nodes. Through performing cox regression, Kaplan-Meier analysis, nomogram, ROC curve, and risk score model, significant prognostic factor was gradually identified. Finally, gene set enrichment analysis (GSEA) analysis was performed to reveal the potential mechanism.ResultsTotally, 74 DEGs were detected, which mainly correlated with infectious disease, signaling molecules and interaction. The 5 important DEGs were filtered, and cox regression further showed that prominin-1 (PROM1) and C-C Motif Chemokine Ligand 2 (CCL2) were prognosis-related factors. A negative correlation was observed between the expression of these 2 genes and the overall survival of metastatic BRCA patients. Especially, PROM1 presented a better prognostic performance on the survival probability of patients. Prognosis verification analysis also confirmed the significant influence of PROM1 on patient’s survival. In addition, we found that PROM1 expression was related to the distant metastasis-free survival in BRCA. Finally, GSEA analysis revealed that PROM1 was negatively related to IGF1 and mTOR pathways involved in BRCA metastasis. ConclusionPROM1 was identified as an important DEGs associated with BRCA bone metastasis. It acted as a vital prognostic biomarker involved in BRCA metastasis, which may be due to the negative regulation of IGF1 and mTOR pathways.