Connecting Three Detection Methods To Screen Polyhydroxyalkanoicates Producing Bacteria In General Nature Region

Abstract This study combined the media containing the fluorescent dye Nile red identification method, the genetic detection method of colony polymerase chain reaction (PCR), and gas chromatography (GC) analysis techniques for screening polyhydroxyalkanoates (PHAs) producers isolated from the natural area in Taiwan. Using the three analysis methods can be schemed into different standard operating procedures to complement each other's limitations and shortcomings. We highlight the development of an economic and efficient screening tool that fits each cost process to different environment samples and intensifies the effective discovery of potent PHAs sources. When screening up to 105 samples to be analyzed, the scheme of connecting three detection methods was reduced to 97% of the cost than using the combination of two methods of Nile red + GC or PCR + GC methods. Besides, the unit of time consumed was only 6%. With the number of samples over 108, the researchers need to consider the overall cost, and only the combination of Nile red and PCR methods without GC will lower the cost to 88% and 57% of the time rather than using all three methods, with the true positive rate of 30.7%.