Internal Transcribed Spacer Region Typing of Trichophyton interdigitale Isolated from Japanese Patients

Trichophyton interdigitale , an anthropophilic species, is one of the main causative agents of tinea unguium and tinea pedis. T. interdigitale and the zoophilic species T. mentagrophytes are morphologically and physiologically very similar. Isolates of the T. interdigitale/T. mentagrophytes complex from around the world have been classified into more than 10 internal transcribed spacer (ITS) genotypes. In this study, we isolated T. interdigitale from Japanese patients and investigated which ITS type was more common. The ITS regions of 29 clinical isolates of T. interdigitale and one clinical isolate of T. mentagrophytes were sequenced. The phylogenetic analysis of the ITS region sequences revealed that the 29 isolates of T. interdigitale belong to ITS type II of T. interdigitale . The one clinical isolate of T. mentagrophytes was in the same cluster with ITS type II* of T. mentagrophytes . One terbinafine-resistant strain of T. interdigitale also belonged to ITS type II of T. interdigitale .