Rewiring metabolism of Clostridium thermocellum for consolidated bioprocessing of lignocellulosic biomass poplar to produce short-chain esters
Bioresource Technology（2023）
Abstract
•Engineered C. thermocellum compatible with CBP for ester production from poplar.•Discovered minor thioesterase activities of esterases limiting ester production.•Improved ester production by manipulating carbon and electron metabolisms.•Severity of poplar CELF pretreatment was positively correlated with ester biosynthesis.
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Key words
Consolidated bioprocessing,Co-solvent enhanced lignocellulosic fractionation,Clostridium thermocellum,Ethanol,Isobutanol,Short-chain ester,Ethyl acetate,Ethyl isobutyrate,Isobutyl acetate,Isobutyl butyrate
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