Demultiplexing Nanopore reads with LAST v7
crossref（2021）
Abstract
This protocol is for a semi-manual method for read demultiplexing, as used after my presentation Sequencing DNA with Linux Cores and Nanopores to work out the number of reads captured by different barcodes. Input: reads as a FASTQ file, barcode sequences as a FASTA file Output: reads split into single FASTQ files per target [barcode] Note: barcode / adapter sequences are not trimmed by this protocol
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