Single Cell Isolation of Human Lung Organoids v1

This protocol describes the single cell isolation of infected human lung organoids in order to perform single cell RNA sequencing or flow cytometry. The protocol focussed on the detailed preparation of samples to prepare single cells for further processing according to the protocols from 10x Genomics for single cell sequencing. The described steps also include the optional fixation of single cells with methanol. In that case, the protocol describes how to rehydrate single cell after methanol fixation to process them for single cell sequencing.