RNA Extraction and RT-qPCR of Human Lung Organoids v1

This protocol describes the RNA extraction from human lung organoids followed by the perfomance of a RT-qPCR to quantify the amount of hostfactors of SARS-CoV-2. The protocol contains detailed steps for the lysis of human lung organoids, as wells as the RNA extraction followed by the reverse transcription into cDNA. In the next section the protocol describes in detail how to quantify the gene expression with the TaqMan gene expression kit and how to calculate the Ct values.