Up-regulated miR-224-5p can target the expression of Neuritin in hearing loss

Abstract Objective Hearing loss is a common neurodegenerative disease and few studies on miRNAs associated with hearing loss. This study screened the differentially expressed miRNAs in hearing loss and explored the relationship between Neuritin and hearing loss at the same time. Methods The combination of kanamycin sulfate and furosemide was used to establish a mouse hearing loss model. High-throughput sequencing was used to screen the differentially expressed miRNAs during hearing loss. ABR was used to detect listening function. QRT-PCR was used to identify the expression of differential miRNAs in hearing loss. Western blot was used to detect the expression of Neuritin protein. Luciferase was used to identify the binding sites of miRNA and Neuritin. Results Neuritin expression decreases after hearing loss. 24 up-regulated miRNAs and 66 down-regulated miRNAs were screened by sequencing. The expression of miR-224-5p increased in the Corti after hearing loss (p < 0.05). MiR-224-5p can inhibit expression of Neuritin and specifically bind to neuritin gene (p < 0.05). Conclusion Up-regulated miRNA-224-5p in hearing loss can target the expression of Neuritin.