CYBA C242T polymorphism is a risk factor for pre-eclampsia:a case control study

Abstract Background: Although the mechanisms responsible for pathogenesis of preeclampsia(PE) have not been entirely clarified, oxidative stress are thought to be its leading cause. As a major component responsible for ROS production during oxidative stresses, p22phox, encoded by CYBA, is one essential subunit of Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase. The aim of this study is to investigate whether CYBA expression and its polymorphism were associated with PE. Methods: The expression of CYBA was analyzed in placenta of PE and control group, as well as in the HTR-8 / Svneo cells stimulated with CoCL2 and TNF-α, respectively. Then, the CYBA C242T polymorphism in 1184 patients with PE and 1421 healthy controls was genotyped by TaqMan probe, and the different distributions were confirmed by a case-control association study. Results: The expression of CYBA mRNA and protein in placenta of pregnant women with PE increased significantly. The expression of CYBA mRNA was also increased in HTR-8/Svneo cells, collected after 24 hours separately stimulated by cobalt chloride and TNF-α. In addition, there was no significant difference in the distribution of C242T locus genotype and allele frequency of CYBA between the case group and the control group (P>0.05). Conclusions: CYBA may play a role in the pathogenesis of oxidative stress in PE, in which it may function via cooperating with TNF-a related inflammatory pathway. Although there was no discrepant distribution of CYBA C242T polymorphism in Chinese population, it is necessary to perform the genetic studies on more CYBA SNPs for multiple regions to gain further insights into its pathogenesis.