Histological and histomorphometrical evaluation of Bio-Oss, Cerabone, and i-PRF for bone regeneration in critical bone defects: An in vivo study

Abstract Objectives: This study aimed to evaluate the bone regeneration performance of the xenografts Bio-Oss and Cerabone, isolated and associated with i-PRF, in critical bone defects in rat calvaria. Materials and Methods: Surgical defects were performed and filled with different materials, according to the referred groups: clot (CG), autogenous bone (AG), Bio-Oss (BO), Cerabone (CB), i-PRF homogenous (i-PRF), Bio-Oss associated with i-PRF (BOPRF) and Cerabone associated with i-PRF (CBPRF). The animals were euthanized for histological and histomorphometric analysis after 4 and 8 weeks. Statistical analysis for bone neoformation assessment was performed by ANOVA and complemented by Tukey's test. Results: The AG group exhibited the highest mean for bone neoformation (37.83 ± 7.96) in this study. Among the bone substitutes, CBPRF group (18.79 ± 5.98) exhibited highest means (p<0.05) compared to BO group (10.20 ± 2.82) and CG group (6.96 ± 3.29). i-PRF group (17.07 ± 4.95), BOPRF group (16.86 ± 6.14), BO group (10.20 ± 2.82), and CB group (16.15 ± 4.72) were not significant among them (p>0.05). Conclusions: According to the results obtained in this study, it was observed that Cerabone® and Bio-Oss®, associated with i-PRF, exhibited a satisfactory applicability to fill critical defects, favoring the bone regeneration process. Clinical Relevance: To the authors' best knowledge, no study in the literature has been found so far, in animals or humans, that compares and associates the materials cited in this study.