DNA metabarcoding protocol for siphonophore gut contents v2

crossref（2022）

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摘要

Protocol for the SiphWeb DNA metabarcoding of siphonophore gut content. Starting with DNA extractions from pooled frozen gastrozooids, this protocol takes the user through the steps of PCR amplification, PCR cleanup and pooling of amplicons from six complementary 18S barcodes that can be submitted into an Illumina MiSeq Lane.

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