Mechanism Exploration of Youth Ischemic Stroke by Metabolomics Analysis

Abstract The onset age of acute ischemic stroke (IS) is gradually younger, which brings great challenges and burden to the medical system. For stroke patients, time is the brain cells, so any method contributes to rapid identification or diagnosis of stroke is extremely meaningful. Metabolomics analysis provides us with new means and ideas for discovering potential stroke-related biomarkers and understanding the pathogenesis of youth IS. In this study, fifty-five patients with IS were assigned in two groups(senior or junior) according to the age(60-year-old) and were performed serum metabolomics analysis based on Liquid Chromatography coupled to tandem Mass Spectrometry (LC-MS) system. Forty differential metabolites were selected, mainly ceramides, lipids, and amino acids, which are mainly related to aging, cardiovascular and cerebrovascular diseases, and antitumor mechanisms. The pathway analysis showed that the differential metabolites were mainly enriched in sphingolippid metabolism, glycine metabolism, serine and threonine metabolism, glycerol phospholipid metabolism, biosynthesis of unsaturated fatty acids, linoleic acid metabolism, and vitamin B6 metabolism. Our study suggests that the mechanism of IS in young adults was most likely associated with elevated plasma lactose ceramide levels and abnormal sphingolipid metabolic pathway.