Dual fluorescent labeling of GLP-1R in live cells via enzymatic tagging and bioorthogonal chemistry

Tracey M. Lewandowski,Peng An,Carlo P. Ramil,Ming Fang,Qing Lin

RSC Chemical Biology（2022）

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摘要

To study GPCR conformational dynamics in live cells, here we report an integrated approach combining enzymatic SNAP-tagging with bioorthogonal chemistry for dual fluorescent labeling of GLP-1R.

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