The role of SIRT1 level and SIRT1 (rs3818292, rs3758391, rs7895833) gene polymorphisms in patients with optic neuritis and multiple sclerosis

Abstract The aim: To investigate the role of SIRT1 level and SIRT1 (rs3818292, rs3758391, rs7895833) gene polymorphisms in patients with optic neuritis (ON) and multiple sclerosis (MS).Methods: We studied 79 patients with ON and 225 healthy subjects. We divided ON patients into 2 subgroups: patients with MS (n=30) and patients without MS (n=43). 6 ON patients did not have sufficient data for MS diagnosis and were excluded from subgroup analysis. The subjects' DNA was extracted from peripheral blood leukocytes and genotyped by real-time polymerase chain reaction. Results were analyzed using the program "IBM SPSS Statistics 26.0".Results: We discovered that SIRT1 rs3758391 was associated with a 2-fold increased odds of developing ON under the codominant (p=0.007), dominant (p=0.011), and over-dominant (p=0.008) models. Also, it was associated with a 3-fold increased odds ofON with MS development under the dominant (p=0.010), 2-fold increased odds under the over-dominant (p=0.032) models and a 1.2-fold increased odds of ON with MS development (p=0.015) under the additive model. We also discovered that the SIRT1 rs7895833 was significantly associated with a 2.5-fold increased odds of ON development under the codominant (p=0.001), dominant (p=0.006), and over-dominant (p<0.001) models, and a 4-fold increased odds of ON with MS development under the codominant (p<0.001), dominant (p=0.001), over-dominant (p<0.001) models and with a 2-fold increased odds of ON with MS development (p=0.013) under the additive genetic model. There was no association between SIRT1 levels and ON with/without MS development.Conclusions: We discovered that the SIRT1 rs3758391 and rs7895833 polymorphisms are associated with ON and ON with MS development.