Abstract 5566: Evaluation of the efficacy of ADC in vitro and in vivo

Abstract Antibody drug conjugate (ADC) is a promising complex with therapeutic potential that aimed to the treatment of solid tumor and hematological malignancies. Compared with the therapeutic monoclonal antibody, ADCs-derived monoclonal antibody is conjugated with cytotoxic agents which can deliver potent cellular toxins to targeted cancer cells specifically. According to the specialization of ADCs, we have established the state-of-the-art platform to support the evaluation of the efficacy of ADC or the combination strategy of ADC and other anti-cancer therapy in the process of pre-clinical drug discovery. such as target validation, efficient internalization, cytotoxic effects and in vivo efficacy testing. In order to explore the mechanism of action and the functional effects of ADC for supporting the application of investigational new drug. Our flow cytometry platform (BD LSRFortessa X20) supports to evaluate the target validation and the process of internalization in a quick, reliable and reproducible way. Also we are able to construct customized antigen-specific overexpression or KI cells used for the development of ADCs which target on the specific antigens theoretically, for example, Claudin18.2 overexpressed MIA-PaCa-2 cell line. In terms of in vivo ADCs assessment, our abundant CDX and PDX resource facilitates the process of in vivo ADCs evaluation, and we have already assess the efficacy of ADC or the combination treatment of ADC and other anti-tumor drugs in several animal models. Last but not the least, our high-throughput MSD (MESO QuickPlex) and Luminex (Bio-Plex࣪ 200 system) platform boosts the evaluation of ADCs safety by measuring the extent of cytokine release syndrome. Citation Format: Jun Huang, Xiaohe Shi, Qingyang Gu, Qunsheng Ji. Evaluation of the efficacy of ADC in vitro and in vivo [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 5566.