Stratification analysis reveal associations between CYP2B6 polymorphisms and lung cancer susceptibility in Chinese Han population

Abstract Background: Recently, many clinical studies have demonstrated that Cytochrome P450 (CYP) is related to the occurrence and development of many cancers. The purpose of our study is to clarify the relevance of CYP2B6 single nucleotide polymorphisms (SNPs) to lung cancer susceptibility.Methods: Five SNPs in CYP2B6 were genotyped in the Han population of China including 507 cases and 505 controls using Agena MassARRAY. Using odds ratio, 95% confidence interval, and χ2 test to calculate the relationship between the SNPs and LC susceptibility. The SNP-SNP interactions were analyzed by performing multifactor dimensionality reduction (MDR).Results: We found that rs2099361 was associated with an increased susceptibility to LC in the codominant model (OR = 1.31, p = 0.045). After stratification analysis, the results found that CYP2B6 rs4803418 (BMI > 24 kg/m2), rs4803420 (BMI > 24 kg/m2, No-smoking), rs12979270 (BMI > 24 kg/m2), and rs1038376 (III-IV versus I-II) were significantly associated with decreased susceptibility of LC. Inversely, rs4803418 (BMI > 24 kg/m2), rs4803420 (III-IV versus I-II) and rs12979270 (No-smoking) showed increased susceptibility to LC. Furthermore, the result of MDR showed that a synergistic relationship might exist between rs12979270, rs4803420, rs2099361, rs1038376, and rs4803418 in CYP2B6 gene and the pathophysiology and genetics of LC. Conclusions: The results suggest that SNPs of CYP2B6 have crucial roles in LC susceptibility, and its correlation is related to the patients’ BMI, smoking status, and clinical stages.