Fluorescent Probe combined with Novel Rapid Microbial Analyzer for Detection of Contaminated Pathogen in Coconut

Abstract Food safety is facing great challenges for preventing foodborne diseases caused by pathogenic pollution, especially in resource-limited area. The rapid detection technique of microorganisms, such as immunological method and molecular biological method, plays a crucial key in the timely bioanalysis and disease treatment strategies. However, it is difficult for these methods to simultaneously meet the criteria of simple operation, high specificity and sensitivity, as well as low cost. Herein, we introduced a Rapid Microbial Analyzer (RMA) that can provide a strong signal with high signal-to-noise ratio via embodying the metabolic features of microbial enzyme target and selective fluorescent substrate. The technique is confirmed high accuracy for the detection of E. coli in the coconut water with a limit of detection of 1 CFU/mL, and could severe as an acute pathogenic detection tool. This device provides a one-step strategy for bacterial detection and is a great leap in the development of easy to operate instrumentation, and could be a great aid for global pathogenic monitoring.