Germline mutation rate in guppies (Poecilia retuculata) confirms a low mutation rate in teleost fishes
Authorea (Authorea)(2022)
Abstract
Mutations are the primary source of all genetic variation. Knowledge about their rates is critical for any evolutionary genetic analyses, but for a long time, that knowledge has remained elusive and indirectly inferred. In recent years, parent-offspring comparisons have yielded the first direct mutation rate estimates. Here, we estimated the mutation rate for the guppy (Poecilia reticulata), a model species in ecoevolutionary studies. We sequenced 4 parents and 20 offspring and screened their genomes for de novo mutations. The initial large number of candidate de novo mutations was analysed with a supervised machine learning approach to remove false-positive results. Additionally, candidate de novo mutations were validated using Sanger sequencing, and positively verified variants were used to estimate the mutation rate. The guppy mutation rate (µ = 3.44 × 10-9 per site per generation) is among the lowest directly estimated mutation rates in vertebrates. Similarly, low estimates were obtained for two other teleost fishes. We discuss potential explanations for such a pattern, as well as the utility of machine-learning approaches for standardized across experiments approach to estimate mutation rates.
MoreTranslated text
Key words
germline mutation rate,low mutation rate,fishes,guppies
AI Read Science
Must-Reading Tree
Example
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined