苦参碱脂质体凝胶的含量测定
Journal of Hubei University of Science and Technology(Medical Sciences)(2020)
Abstract
目的 建立苦参碱脂质体凝胶中苦参碱的含量测定方法.方法 采用高效液相色谱法,C18柱(250mm ×4.6mm,5μmol/L),以乙腈-水(9:1)为流动相,检测波长为212nm,流速0.8mL/min,柱温为20℃,对苦参碱脂质凝胶中的苦参碱进行含量测定.结果 苦参碱在1.0~80.0μg/mL范围内有良好的线性关系,相关系数为0.9996,回收率98.24%.结论 该测定方法重复性好,精密度良好,可用于苦参碱脂质体凝胶中苦参碱的含量测定.
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