Sulfones of Pyridinyloxy-Substituted Imidazo[2,1-b][1,3]thiazines: Synthesis, Anti-Inflammatory Activity Evaluation In Vivo and Docking Studies

Background Heterocyclic sulfone-bearing small molecules are particularly important objects in medicinal chemistry. Structure-diversified pyridinyloxy-substituted imidazo[2,1-b][1,3]thiazines are characterized by satisfactory drug-like parameters and possess significant anti-inflammatory effects in in vivo studies. Objective Oxidation of a series of 6-(2-pyridinyloxy) imidazo[2,1-b][1,3]thiazines under the action of m-chloroperbenzoic acid with a view to anti-inflammatory activity enhancement and structure optimization. Methods A series of appropriate sulfones was synthesized by the action of m-chloroperbenzoic acid on 6-[(pyridin-2-yl)oxy]-6,7-dihydro-5H-imidazo[2,1-b][1,3]thiazines. The structure of the synthesized products was confirmed by H-1, C-13 NMR, and LC-MS spectra. In vivo anti-inflammatory activity was studied using carrageenin model of inflammatory oedema on white rats. ADMET parameters of compounds were evaluated in silico using AdmetSAR. For the most active compounds, docking studies to COX-1,2, 5-LOX and FLAP were performed. Results It was found that transformation to sulfones could be achieved by soft oxidation at room temperature for 48 h using the 3-fold excess of oxidant in the presence of sodium hydrogen phosphate. Anti-inflammatory activity screening results revealed that all synthesized sulfones showed significant anti-exudative action with inflammation inhibition index in the range of 37.7 - 48.1%. The compound 2i was found to be the most active in the experiment, and its activity was equal to the reference drug effect; it also possesses satisfactory ADMET parameters and high energy of binding to 5-LOX and FLAP. Conclusion The synthesized sulfone 2i is of interest for in-depth studies and further design of new potential non-steroidal anti-inflammatory agents.