Antimicrobial activity of candidate probiotic Streptococcus salivarius against Gram-positive bacteria in oral cavity

Objectives:The aim of this study is to investigate antimicrobial activity in isolated Streptococcus salivarius against Gram-positive bacteria related oral diseases.Methods: S. salivarius was used in G2, G7, K12, and ATCC 7073 strains and tryptic soy broth supplemented with glucose was cultivated.Actinomyces israelii, Actinomyces viscosus, and Enterococcus faecalis were cultivated with brain heart infusion broth.Streptococcus mutans and Streptococcus sobrinus were maintained using tryptic soy broth.The antimicrobial activity of S. salivarius was performed by minimum inhibitory concentration using the spent culture medium.Results: All S. salivarius have antimicrobial activity against Gram-positive bacteria in oral cavity.When comparing antimicrobial activity, S. salivarius G2 and G7 as isolated strain showed stronger antimicrobial activity against Gram-positive microbe than type K12 strain.Conclusions: S. salivarius G2 and G7 have strong antimicrobial activity and may be prevent oral disease by Gram-positive bacteria in oral cavity.